Genetic analysis of androgenetic traits in wheat [Triticum aestivum L.]

The objective of this study was to estimate genetic parameters and to investigate the type of gene action in controlling androgenesis in wheat. Two wheat cultivars of Grebe and Houtman were reciprocally crossed with two synthetic genotypes of Do1 and Pol and then a complete set of the parents, F1, reciprocal F1 [RF1], F2 and back-cross generations [BC1 and BC2] of each cross were used for anther culture. The ratio of responding anthers, the ratio of albino and green regenerants, and the number of embryoids per each responding anther were determined for different generations of each cross. The results showed a wide genetic variation for embryoid induction and plant regeneration among the parental lines and their progenies. The genetic model of additive-dominance effects could explain the variation among the generation means for the traits, indicating that their inheritance was relatively simple. The genetic analysis also showed predominance of additive genetic effects in genetic control of embryoid induction and green plant regeneration, implying possible improvement of these traits by selection in plant breeding programs. Maternal effects were also found for embryoid induction. The narrow-sense heritability for responding anthers, green plants, albino plants, green plants to total regenerants, and embryoids per responding anther in different crosses varied from 41% to 77%, 64% to 92%, 67% to 84%, 40% to 67% and 33% to 65%, respectively. In conclusion, it seems that the improvement of green plant regeneration in another culture technique can be achieved by appropriate breeding and selection programs

Insensibilidad total a los andrógenos con persistencia de derivados müllerianos: reporte de un caso / Complete audiogen insensitivity with coexistence of mullerian derivates: report a case

Presentamos el caso de una paciente de 25 años de edad con insensibilidad total a los andrógenos, quien fue referida a nuestro centro por no haber presentado menarquia ni desarrollo de caracteres sexuales secundarios. Al examen físico se observó falta de vello axilar y pubiano, volumen mamario normal y vagina corta terminada en fondo de saco. El estudio genético reveló cariotipo masculino normal. Se le practicó una laparotomía que evidenció la presencia de un útero con una trompa de Falopio derecha, ambos hipoplásicos, y un testículo derecho normal. En vista de que la presencia de derivados müllerianos coexistiendo con insensibilidad completa a los andrógenos es poco frecuente, traemos a consideración el presente caso clínico

Molecular analysis of androgen resistance syndromes in Egyptian patients

This study aimed to examine AR and 5 alpha-reductase 2 [5 alpha R2] gene mutations among a sample of such cases as a first step towards instituting a screening program. Five families with a typical hormonal profile of 5 alpha RD were screened for major deletions of exons 3-5 of the 5 alpha R2 gene using polymerase chain reaction [PCR] and electrophoresis. Consequently, screening for point mutations was carried out using single strand conformational polymorphism [SS CP] analysis, followed by nucleotide sequencing. Likewise, seven patients with androgen insensitivity syndrome [AIS] were subjected to molecular analysis of AR exons B-H along the same scheme, except for the use of denaturing gradient gel electrophoresis [DGGE] for screening point mutations. The study showed the absence of major deletions in either gene. One family had abnormal electrophoretic mobility on SSCP of exon 5 of the 5 alpha R2 gene resulting from a point mutation of C to T substitution at codon 246. Another family showing retarded mobility on DGGE had a point mutation of G to A substitution at codon 889 of the AR gene. The study revealed two mutations that were previously reported in other geographically distinct populations inferring the possibility of the presence of mutational host spots in the genes